The Difference of Subcellular Localization betweenβ-Conglycinin and Glycinin in Developing Soybean Cotyledons
نویسندگان
چکیده
منابع مشابه
beta-Conglycinin and glycinin in high-protein soybean seeds.
The agronomic performance and storage proteins of high seed protein lines of soybeans [Glycine max L. (Merr.)] were investigated to determine if the two major storage proteins, beta-conglycinin and glycinin, contribute to the increased protein content of high seed protein lines. Subunits of these two major storage proteins were estimated by scanning SDS-PAGE gels by scanning densitometry. The r...
متن کاملDifferential Proteolysis of Glycinin and beta-Conglycinin Polypeptides during Soybean Germination and Seedling Growth.
The degradation of the major seed storage globulins of the soybean (Glycine max [L.] Merrill) was examined during the first 12 days of germination and seedling growth. The appearance of glycinin and beta-conglycinin degradation products was detected by sodium dodecyl sulfate-polyacrylamide gel electrophoresis of cotyledon extracts followed by electroblotting to nitrocellulose and immunostaining...
متن کاملAssessing Glycinin (11S) and b-Conglycinin (7S) Fractions of Soybean Storage Protein by Near-Infrared Spectroscopy
Soybean breeding programs underway today are addressing the goal of improving the protein profile to benefit the human diet as well as that of livestock. Glycinin, a globulin storage protein of the meal and designated as the 11S size fraction by ultracentrifugation, is desirable because of its relative abundance of sulfur-containing amino acids, such as methionine and cysteine. The current stud...
متن کاملArginine Metabolism in Developing Soybean Cotyledons
Tracerkinetic experiments were performed using L-[guanidino14C]arginine, L-[U-14C]arginine, L-[ureido-14C]citrulline, and L-[114C]omithine to investigate arginine utilization in developing cotyledons of Glycine max (L.) Merrill. Excised cotyledons were injected with carrier-free 14C compounds and incubated in sealed vials containing a CO2 trap. The free and protein amino acids were analyzed usi...
متن کاملElectrogenic sucrose transport in developing soybean cotyledons.
Addition of sucrose to a solution bathing an excised developing soybean cotyledon causes a transient depolarization of the membrane potential, as measured using standard electrophysiological techniques. The magnitude of the depolarization is dependent on the concentration of both sucrose and protons in a manner which suggests carrier mediation; this process has an apparent K(m) for sucrose of a...
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ژورنال
عنوان ژورنال: Agricultural and Biological Chemistry
سال: 1988
ISSN: 0002-1369
DOI: 10.1080/00021369.1988.10868726